Imm-Link (TM) KLH (Carboxyl) Immunogen labeling Kit

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Summary

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Imm-Link (TM) KLH (Carboxyl) Immunogen labeling Kit Summary

Description
BSA, Ovalbumin or Keyhole Limpet Hemocyanin (KLH) stimulate a large immune response and are typically used as carrier proteins to generate antibodies against small antigens (peptides or organic compounds). For success the antigen (normally referred to as a hapten) needs to be covalently linked to the carrier protein.

The imm-Link carboxyl conjugation kit allows carboxyl containing haptens to be conjugated to a carrier protein simply by adding a solution of the hapten to a proprietary lyophilised mixture containing the carrier protein and all the required conjugation chemistry. The hands-on time to set up the conjugation reaction is typically 20-30 seconds.

Following successful conjugation the conjugate is dialysed in the supplied dialysis cartridge to remove unwanted by products. The hapten conjugate can then be easily recovered and used.

Immunogen Kit Carboxyl
Target Functional Groups Glutamic Acid, Aspartic Acid or free carboxyl groups


Features:
  • Easy to use
  • All components supplied
  • Wide range of chemistries
  • Hands on time to set up conjugation reaction is 20-30 seconds
  • Supplied dialysis cartridge ensures conjugates are recovered in high yield
  • Full technical support available
Kit Type
Immunogen labeling Kit

Applications/Dilutions

Application Notes
Upon dissolution of the Imm-Link mixture, proprietary chemicals in the mixture become activated, resulting in the coupling of the hapten to the carrier protein in a gentle and controlled process. The hands-on time to set up the conjugation reaction is typically 20-30 seconds
Publications
Read Publication using 470-0500.

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. 1 or 3 vial(s) of Imm-Link Carboxyl Modifier reagent
  2. 1 or 3 dialysis cartridge(s)
  3. 1 or 3 glass vial(s) of Imm-Link mix
  4. 1 or 3 250 ml bottle(s) of 10x dialysis buffer

Notes

Imm-Link® is a registered trademark of Expedeon Inc. and distributed by Novus Biologicals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Imm-Link (TM) KLH (Carboxyl) Kit (470-0500)(1)


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