Imm-Link™ KLH (Amine) Immunogen labeling Kit

Product Discontinued

Imm-Link™ KLH (Amine) Immunogen labeling Kit Summary

• Description: BSA, Ovalbumin or Keyhole Limpet Hemocyanin (KLH) stimulate a large immune response and are typically used as carrier proteins to generate antibodies against small antigens (peptides or organic compounds). For success the antigen (normally referred to as a hapten) needs to be covalently linked to the carrier protein.
  
  The imm-Link amine conjugation kit allows amine containing haptens to be conjugated to a carrier protein simply by adding a solution of the hapten to a proprietary lyophilised mixture containing the carrier protein and all the required conjugation chemistry. The hands-on time to set up the conjugation reaction is typically 20-30 seconds.
  
  Following successful conjugation the conjugate is dialysed in the supplied dialysis cartridge to remove unwanted by products. The hapten conjugate can then be easily recovered and used.
  
  

  Immunogen Kit	Amine
  Target Functional Groups	Lysines or free amines NH2

  
  
  Features:
  • Easy to use
  • All components supplied
  • Wide range of chemistries
  • Hands on time to set up conjugation reaction is 20-30 seconds
  • Supplied dialysis cartridge ensures conjugates are recovered in high yield
  • Full technical support available
• Kit Type: Immunogen labeling Kit

Applications/Dilutions

• Application Notes: Upon dissolution of the Imm-Link mixture, proprietary chemicals in the mixture become activated, resulting in the coupling of the hapten to the carrier protein in a gentle and controlled process. The hands-on time to set up the conjugation reaction is typically 20-30 seconds

Packaging, Storage & Formulations

• Storage: Store at -20C.

Notes

Imm-Link® is a registered trademark of Expedeon Inc. and distributed by Novus Biologicals.

Background

The Imm-Link KLH (Amine) conjugation kit allows amine containing haptens to be conjugated to Keyhole Limpet Hemocyanin (KLH) by simply adding a solution of the hapten to a proprietary lyophilised mixture containing KLH, the carrier protein, and all the required conjugation chemistry. KLH stimulates a large immune response and is typically used as a carrier protein to generate antibodies against small antigens (peptides or organic compounds). For success the antigen (normally referred to as a hapten) needs to be covalently linked to the carrier protein, in this case, KLH.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Imm-Link (TM) KLH (Amine) Kit (450-0500)(1)

Publication using 450-0500

• Gurale BP, He Y, Cui X et al. Bioconjugate Chemistry: Towards the Development of next generation of Rapid Diagnostic Test: Synthesis of Glycophosphatidylinositol (GPI) analogues of Plasmodium falciparum and immunological characterization. Bioconjug Chem 2016-12-21 [PMID: 27792303] (Rabbits)