BSA, Ovalbumin or Keyhole Limpet Hemocyanin (KLH) stimulate a large immune response and are typically used as carrier proteins to generate antibodies against small antigens (peptides or organic compounds). For success the antigen (normally referred to as a hapten) needs to be covalently linked to the carrier protein.
The imm-Link amine conjugation kit allows amine containing haptens to be conjugated to a carrier protein simply by adding a solution of the hapten to a proprietary lyophilised mixture containing the carrier protein and all the required conjugation chemistry. The hands-on time to set up the conjugation reaction is typically 20-30 seconds.
Following successful conjugation the conjugate is dialysed in the supplied dialysis cartridge to remove unwanted by products. The hapten conjugate can then be easily recovered and used.
Immunogen Kit
Amine
Target Functional Groups
Lysines or free amines NH2
Features:
Easy to use
All components supplied
Wide range of chemistries
Hands on time to set up conjugation reaction is 20-30 seconds
Supplied dialysis cartridge ensures conjugates are recovered in high yield
Full technical support available
Kit Type
Immunogen labeling Kit
Applications/Dilutions
Application Notes
Upon dissolution of the Imm-Link mixture, proprietary chemicals in the mixture become activated, resulting in the coupling of the hapten to the carrier protein in a gentle and controlled process. The hands-on time to set up the conjugation reaction is typically 20-30 seconds
Imm-Link® is a registered trademark of Expedeon Inc. and distributed by Novus Biologicals.
Background
The Imm-Link KLH (Amine) conjugation kit allows amine containing haptens to be conjugated to Keyhole Limpet Hemocyanin (KLH) by simply adding a solution of the hapten to a proprietary lyophilised mixture containing KLH, the carrier protein, and all the required conjugation chemistry. KLH stimulates a large immune response and is typically used as a carrier protein to generate antibodies against small antigens (peptides or organic compounds). For success the antigen (normally referred to as a hapten) needs to be covalently linked to the carrier protein, in this case, KLH.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Publications for Imm-Link (TM) KLH (Amine) Kit (450-0500)(1)
We have publications tested in 1 confirmed species: Rabbits.
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