Imidazole ELISA Kit (Colorimetric) Summary
| Description |
Imidazole ELISA Kit is a competitive binding enzyme immunoassay for the quantitative measurement of imidazole. |
| Sensitivity |
The actual detection limit is based on the setup of experiment protocol, such as extraction procedure and dilution factor. |
| Assay Type |
Competitive ELISA |
| Sample Volume |
50 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
| Dilutions |
- Competitive ELISA
- ELISA
- Quantification
|
| Publications |
|
Packaging, Storage & Formulations
Kit Components
|
Components
|
- (#3) Imidazole-Alkaline Phosphatase conjugate (IDZ-ALP).
- (#5) p-Nitrophenyl Phosphate (pNPP) substrate. Ready to use.
- (#6) Wash Buffer (10xPBS-Tween). Dilute 10 fold with distilled or deionized water to150 mL prior to use.
- (#7) Stop Solution, 3N NaOH.
- 96-wells microtiter plate (#S). Twelve strips of 8 detachable wells, coated withAnti-Imidazole antibody.
- Calibrator containing 0, 1.0, 3.0 and 9.0 µg/mL of imidazole.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
Publications for Imidazole ELISA Kit (KA1421)(1)
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Product General Protocols
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FAQs for Imidazole ELISA Kit (KA1421). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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