IkBa Mutant Adenovirus Assay Kit

Product Discontinued

IkBa Mutant Adenovirus Assay Kit Summary

• Description: IkBa dominant mutant (IkBaM) is a mutated form of IkBa with a serine-to alanine mutations at residues 32 and 36 (8). Overexpression of IkBaM presumably prevents phosphorylation of native IkBa and there by inhibiting translocation of NF-kB complex to the nucleus and thereby preventing NF-kB activation. In most cell lines, the inhibition of NF-kB activation affects cell growth. The IkBaM was tested on proliferation of HeLa cells in tissue culture plates.
• Kit Type: Assay Kit

Applications/Dilutions

Publications

Read Publications using NBP2-29443 in the following applications: WB 1 publication

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.

Notes

Human IkBa deletion mutant adenovirus. Addition of this virus to cell culture inhibits NF-kB activation. The virus is replication incompetent in non-permissive host cells due to the deletion of genes required from viral replication.

Alternate Names for IkBa Mutant Adenovirus Assay Kit

• IkB alpha Mutant Adenovirus
• IkBalpha Mutant Adenovirus

Background

NF-kB is controlled by a family of inhibitory proteins called, IkBs (1). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/B, and IKK3/y (2-5). External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to the activation of IkB-kinase complex that specifically phosphorylates IkBa on Serine-32 and Serine-36. Phosphorylation of these sites leads to ubiquitination of IkBa and subsequent degradation by the 26 S proteasome. Degradation of IkBa results in unmasking of the nuclear localization signal of NF-kB dimers, which subsequently translocates to the nucleus and activates target genes (6,7). Thus analysis of the phosphorylation of IkBa has a correlation to the activity of the IKK complex as well as the nuclear localization and activation of NF-kB

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for IkBa Mutant Adenovirus Kit (NBP2-29443)(2)

Publications using NBP2-29443

• Wang Z, Castresana MR, Detmer K et al. An IkappaB-alpha mutant inhibits cytokine gene expression and proliferation in human vascular smooth muscle cells. J Surg Res. 2002-02-01 [PMID: 11796019] (WB, Human)
  
  Details:
  Construction of the IMG-2500 IkBa deletion mutant adenovirus (IkBaM) (Materials and Methods); Primary human vascular smooth muscle cells (VSMC) growing in culture infected with IkBaM and analyzed for IkBaM expression by WB using an IKBa antibody (Fig 1);
• Kusne Y. The Dissection of Signaling Cascades in Neural Stem Cell Proliferation & GBM Promotion Thesis. 2014-04-01
  
  Details:
  IkBaM adenovirus used for induction of various genes in U251/EGFR cells treated with 100 ng/mL EGF for 6 h or 10 ng/mL TNF alpha for 1 h (Figure 3.S13 E and F)