Rabbit anti-Chicken IgY (H+L) Secondary Antibody

• Reactivity: Ch
• Applications: WB, ELISA, Func, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rabbit anti-Chicken IgY (H+L) Secondary Antibody Summary

• Description: Purity > 95% based on SDS-PAGE. Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
• Immunogen: Purified Chicken IgG/Y (H&L)
• Specificity: Based on IEP, this antibody reacts with heavy gamma chains on chicken IgG/Y and light chains on all chicken immunoglobulins
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoassay
  • Immunodiffusion
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100-1:2000
• Application Notes: This antibody is suitable for all immunoassay applications.

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin chicken serum immunoglobulins

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chicken IgG/IgY, H/L Chains Antibody (NBP1-74781)(1)

Publication using NBP1-74781

• Patel AJ, Wan YW, Al-Ouran R et al. Molecular profiling predicts meningioma recurrence and reveals loss of DREAM complex repression in aggressive tumors Proceedings of the National Academy of Sciences 2019-10-22 [PMID: 31591222] (Immunoprecipitation, Western Blot)