Mouse IgM Kappa Light Chain Isotype Control (MMK)

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SDS-Page: Mouse IgM Kappa Light Chain Isotype Control (MMK) [NBP1-96975] - SDS-PAGE of Mouse IgM Kappa isotype control. Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF, PAGE
Clone
MMK
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Purified

Order Details

Mouse IgM Kappa Light Chain Isotype Control (MMK) Summary

Description
Mouse IgM Kappa Light Chain isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated below.
Specificity
Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, Ig3, IgA. Light chain composition has been confirmed by SDS-PAGE.
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Multi-step

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1:1000-1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • SDS-Page
  • Western Blot 1:100 - 1:2000
Application Notes
This isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Preservative
0.1% Sodium Azide
Purity
Multi-step

Notes

This isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum.

Store vial at 4C prior to opening. Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Background

Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and u - IgM, light chain kappa and lambda.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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