Rabbit anti-Mouse IgM (H+L) Secondary Antibody [HRP]

Dot Blot: Rabbit anti-Mouse IgM (H+L) Secondary Antibody [HRP] [NBP1-73885] - Dot Blot results of Rabbit anti-Mouse IgM (H+L) Secondary antibody [HRP].Dots are Mouse IgM at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 30 min at RT.Primary Antibody: none.Secondary Antibody: Rabbit anti-Mouse IgM -HRP at 1ug/mL for 1hr at RT.

• Reactivity: Mu
• Applications: WB, DB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: HRP

Rabbit anti-Mouse IgM (H+L) Secondary Antibody [HRP] Summary

• Description: For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
  
  This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum
• Immunogen: Rabbit anti-Mouse IgM (H+L) Secondary Antibody [HRP] was produced by repeated immunization with mouse IgM whole molecule in rabbit.
• Specificity: This reagent primarily recognizes Mouse IgM however some light chain cross reactivity will occur against other mouse immunoglobulins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:500 - 1:2500
  • Western Blot 1:1000 - 1:10000
• Application Notes: This secondary peroxidase conjugated antibody This product has been tested by dot blot and ELISA and is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Mouse IgM (H&L) peroxidase conjugated antibody has been assayed against 1.0 ug of mouse IgM in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
  
  NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Gentamicin Sulfate
• Purity: Multi-step

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.