Goat anti-Porcine IgM (H+L) Secondary Antibody [HRP]

Product Discontinued

Goat anti-Porcine IgM (H+L) Secondary Antibody [HRP] Summary

• Immunogen: Pig IgM
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with pig IgM. This may cross react with IgM from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-1:10000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS) containing 0.2%BSA
• Preservative: 0.1% Pro-Clean 400
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Porcine IgM Antibody (NB718)(4)

Publications using NB718

• Takahashi M, Nishizawa T, Tanaka T et al. Correlation between positivity for immunoglobulin A antibodies and viraemia of swine hepatitis E virus observed among farm pigs in Japan. J Gen Virol 2005-06-01 [PMID: 15914860]
• Chang KO, Vandal OH, Yuan L et al. Antibody-secreting cell responses to rotavirus proteins in gnotobiotic pigs inoculated with attenuated or virulent human rotavirus. J Clin Microbiol 2001-08-01 [PMID: 11473996]
• Felder CB, Vorlaender N, Gander B et al. Microencapsulated enterotoxigenic Escherichia coli and detached fimbriae for peroral vaccination of pigs. Vaccine 2000-11-01 [PMID: 11115691]
• Huter V, Hensel A, BrE et al. Improved protection against lung colonization by Actinobacillus pleuropneumoniae ghosts: characterization of a genetically inactivated vaccine. J Biotechnol 2000-09-01 [PMID: 11000472]

FAQs for Porcine IgM Antibody (NB718). (Showing 1 - 1 of 1 FAQs).

1. Our customer would like to know if NB718 would react to other porcine serum proteins, such as IgG. If there is any further information about its specificity, please share with us. How about other porcine serum proteins, such as IgG? The customer would like to know if NB718 would cross react with IgM, IgA and he also would like to know if it is made from the full-length porcine IgM, or just part of IgM. The customer still has few more questions: 1. The purification of NB718 is described as Immunogen affinity purified on the product page. The customer would like to know further about how the purification is proceeded.2. Based on its datasheet, this antibody can be used for IHC and ELISA. May we have the QC results of NB718 for the customer's reference? 3. Concerning specificity, what the difference is between NB718 and NBP1-73908?
   • To answer your question, NB718 would react specifically with pig IgM but it may cross react with IgM from other species as well. So NB718 is specific to IgM and would not react with IgG and it was made of heavy chain IgM. With regards to the question No.1, NB718 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP) but in order to provide you with more detailed information on purification process and QC data, I have to contact our lab and I will be in touch with you as soon as I have the answer. NBP1-73908 is an anti-SWINE IgM (mu) antibody that has been generated in rabbit. As far as the specificity is concerned, both antibodies are the same except the host they were generated in.