Rabbit anti-Goat IgM Fc Secondary Antibody [HRP] (Azide Free)

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-69686
    • Availability
      Product Discontinued

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Rabbit anti-Goat IgM Fc Secondary Antibody [HRP] (Azide Free) Summary

Description
The lyophilized conjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Immunogen
Purified normal IgM isolated from pooled goat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Horseradish peroxidase-conjugated IgG fraction of polyclonal Rabbit antiSerum to Goat IgM, Fc specific.
Clonality
Polyclonal
Host
Rabbit
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Dot Blot
  • ELISA
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.2)
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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