Goat anti-Guinea Pig IgM Fc Secondary Antibody [FITC]

Product Discontinued

Goat anti-Guinea Pig IgM Fc Secondary Antibody [FITC] Summary

• Description: The lyophilized conjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
• Immunogen: Purified IgM isolated from guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against guinea pig serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgM used as immunogens. It does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
• Clonality: Polyclonal
• Host: Goat
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry-Frozen
• Application Notes: (In)direct immunofluorescence.

Reactivity Notes

This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this antiserum has not been tested in detail.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Lyophilized from PBS (pH 7.2)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Ion exchange chromatography
• Reconstitution Instructions: Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.