Mouse IgG3 Lambda Isotype Control (MG3L)

SDS-Page: Mouse IgG3 Lambda Isotype Control (MG3L) [NBP1-96977] - SDS PAGE Results of Mouse IgG3 Lambda isotype control (MG3L). Lane 1: Mouse IgG3 Lambda isotype control (MG3L) Reduced [5ug]. Lane 2: Unstained Molecular weight marker. Lane 3: Mouse IgG3 Lambda isotype control (MG3L) Non-Reduced [5ug].4-20% Gel.

• Reactivity: Mu
• Applications: WB, ELISA, Flow, ICC/IF, PAGE
• Clone: MG3L
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Purified

Mouse IgG3 Lambda Isotype Control (MG3L) Summary

• Description: This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.
  
  Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Specificity: Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM
• Isotype: IgG3 Lambda
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:1000-1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • SDS-Page
  • Western Blot
• Application Notes: This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

FAQs for IgG3 Lambda Isotype Control (NBP1-96977). (Showing 1 - 1 of 1 FAQ).

1. Please provide information about Mouse IgG3 Isotype Control (MG3L)(#NBP1-96977). Has the light chain of this Ab been identified? If so, kappa or lambda?
   • About the subclass of this isotype control, the following statements were derived from the databases of our manufacturing lab for the product: Application Note: Mouse IgG3 lambda isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important. Purity/Specifity: Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgM and IgA.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum.  Light and heavy chain composition confirmed by RID. That being said, NBP1-96977 has the lambda light chain, and only reacted with IgG3 itself at the QC tests. Unlike IgG1 and IgG2, which have many subclasses, IgG3 does not (https://absoluteantibody.com/antibody-resources/antibody-overview/antibody-isotypes-subtypes/, https://www.jacksonimmuno.com/technical/products/groups/subclass-specific, https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.0300-9475.2004.01362.x, and others). In summary, NBP1-96977 is a mouse IgG3 Isotype Control (against IgG3 only no subclass) with the lambda light chain.