Rabbit anti-Mouse IgG3 Heavy Chain Secondary Antibody

Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG3 Heavy Chain Secondary Antibody [NBP1-72796] - Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG3 Secondary Antibody [DyLight 488] [NBP1-72893] Image from the DyLight 488 version of this antibody.

Flow Cytometry: Rabbit anti-Mouse IgG3 Heavy Chain Secondary Antibody [NBP1-72796] - Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG3 Antibody [DyLight 405] [NBP1-72923]

Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG3 Heavy Chain Secondary Antibody [NBP1-72796] - Rabbit anti-Mouse IgG3 Antibody [DyLight 800] [NBP1-72917]

• Reactivity: Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rabbit anti-Mouse IgG3 Heavy Chain Secondary Antibody Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum and Mouse IgG.
  
  Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Mouse IgG3 heavy chain
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 - 1:50000
  • Flow Cytometry
  • Fluorophore-linked immunosorbent assay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:1000 - 1:5000
• Application Notes: This product has been tested by ELISA and dot blot and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.