Mouse IgG2b Kappa Isotype Control (M2BK)

Flow Cytometry: Mouse IgG2b Kappa Isotype Control (M2BK) [NBP1-96980] - Cells were stained with the positive antibody (right) and Isotype control (left) and unstained cells for 1h at 4C. Image from verified customer review.

• Reactivity: Mu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: M2BK
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Purified

Mouse IgG2b Kappa Isotype Control (M2BK) Summary

• Description: This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.
  
  Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Specificity: Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgA, IgM
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Reviews for IgG2b Kappa Isotype Control (NBP1-96980) (2)

Average Rating: 5

(Based on 2 reviews)

reviewed by: Verified Customer 02/27/2019

Summary

• Lot: 30448

Comments

• Comments: Cells were stained with the positive antibody (right) and Isotype control (left) and unstained cells for 1h at 4C

reviewed by: Verified Customer 06/28/2018

Summary

• Lot: 30448

Comments

• Comments: antibody staining negative with isotype control