Goat anti-Mouse IgG2b Heavy Chain Secondary Antibody [HRP]

• Reactivity: Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP
• Concentration: 1.0 mg/ml

Goat anti-Mouse IgG2b Heavy Chain Secondary Antibody [HRP] Summary

• Immunogen: Mouse IgG2b
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with mouse IgG2b. This may cross react with IgG2b from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:2000-1:20000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS) containing 0.2%BSA
• Preservative: 0.05% Pro-Clean 400
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG2b Antibody (NB7521)(5)

Publications using NB7521

• Garcia-Castro D, Mazuk J, Heine E et al. Increased SIRT3 combined with PARP inhibition rescues motor function of SBMA mice iScience 2023-07-01 [PMID: 37599829] (WB)
• Dave Rajnish S, Sharma Ravi K, Muir Roshell R et al FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques. J Neuroimmune Pharmacol. 2018-06-01 [PMID: 29288344] (IHC-P)
  
  Details:
  Citation using the HRP version of this antibody.
• Goser S, Ottl R, Brodner A et al. Critical role for monocyte chemoattractant protein-1 and macrophage inflammatory protein-1alpha in induction of experimental autoimmune myocarditis and effective anti-monocyte chemoattractant protein-1 gene therapy. Circulation. [PMID: 16316965]
  
  Details:
  Citation using the HRP form of this antibody.
• Kaya Z, Afanasyeva M, Wang Y et al. Contribution of the innate immune system to autoimmune myocarditis: a role for complement. Nat Immunol. [PMID: 11477411]
  
  Details:
  Citation using the HRP form of this antibody.
• Wang Y, Afanasyeva M, Hill SL et al. Nasal administration of cardiac myosin suppresses autoimmune myocarditis in mice. J Am Coll Cardiol. [PMID: 11092676]
  
  Details:
  Citation using the HRP form of this antibody.