Rabbit anti-Mouse IgG2B Secondary Antibody [DyLight 488]


Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2b Antibody [DyLight 488] [NBP1-72892]

Product Details

Reactivity MuSpecies Glossary
Applications WB, FLISA, ICC/IF
1 mg/ml

Order Details

Rabbit anti-Mouse IgG2B Secondary Antibody [DyLight 488] Summary

Mouse IgG2b chain
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. Minimal cross reactivity was noted against other Mouse immunoglobulin classes or subclasses.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
DyLight 488
DyLight 488 (A=493, E=518)
Lyophilized 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) Immunoglobulin and Protease free
0.01% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:10000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.


Emission Wavelength: 518, Excitation Wavelength: 493, Label Molecular Weight: 1,011, Restore with 0.1 mL of deionized water (or equivalent).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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