Mouse IgG2a Kappa Light Chain Isotype Control (MOPC-173) [Allophycocyanin]

Product Discontinued

Mouse IgG2a Kappa Light Chain Isotype Control (MOPC-173) [Allophycocyanin] Summary

• Description: The MOPC-173 immunoglobulin is of Mouse IgG2a Kappa isotype and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed Mouse, Rat and Human tissues.
• Immunogen: TNP and KLH
• Specificity: The MOPC-173 immunoglobulin has unknown specificity.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Flow: Use 20 ul for a million cells. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 0.2% BSA, PBS, pH 7.2.
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Notes

Endotoxin level < 0.01 ng/ug.

Background

MOPC-173 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical stainingand immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.