Mouse IgG2a Kappa Light Chain Isotype Control (MG2a-53) [PerCP]

Product Discontinued

Mouse IgG2a Kappa Light Chain Isotype Control (MG2a-53) [PerCP] Summary

• Description: The MG2a-53 immunoglobulin is Mouse IgG2a Kappa isotype and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed Mouse, Rat and Human tissues.
• Immunogen: TNP and KLH
• Specificity: Immunoglobulin reacts with Trinitrophenol and Keyhole limpet hemocyanin (KLH).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Useful as an isotype-matched control in WB, IP, IHC-P, IHC-Fr and IF (surface or intracellular) for Flow. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS.
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Endotoxin level < 0.1 EU/ug.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.