Mouse IgG2a Kappa Light Chain Isotype Control (MG2a-53)

Product Discontinued

Mouse IgG2a Kappa Light Chain Isotype Control (MG2a-53) Summary

• Description: The MG2a-53 immunoglobulin is Mouse IgG2a Kappa isotype and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed Mouse, Rat and Human tissues.
• Immunogen: TNP and KLH
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Useful as an isotype-matched control in WB, IP, IHC-P, IHC-Fr and IF (surface or intracellular) for Flow. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS, pH 7.2.
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Notes

Endotoxin level < 0.1 EU/ug.

Background

ab18415 MG2a-53 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2a Kappa Light Chain Isotype Control (NB600-986)(3)

Publications using NB600-986

• Bokelmann M, Vogel U, Debeljak F Et al. Tolerance and Persistence of Ebola Virus in Primary Cells from Mops condylurus, a Potential Ebola Virus Reservoir Viruses 2021-10-29 [PMID: 34834992]
• Bokelmann M, Edenborough K, Hetzelt N et al. Utility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoir PLoS Negl Trop Dis 2020-01-01 [PMID: 31961874] (FLOW, Bat)
• Hoel AW, Yu P, Nguyen KP, et al. Mitochondrial Heat Shock Protein-90 Modulates Vascular Smooth Muscle Cell Survival and the Vascular Injury Response in Vivo. Am J Pathol. [PMID: 22841823] (IHC-Fr)
  
  Details:
  Citation using the Unpurified form of this antibody.