Mouse IgG2a Kappa Isotype Control (M2AK)

SDS-Page: Mouse IgG2a Kappa Isotype Control (M2AK) [NBP1-96981] - SDS-PAGE of Mouse IgG2a Kappa Isotype Control. Lane 1: Mouse IgG2a Kappa Isotype Control, Non-reduced. M: Opal Pre-stained Ladder. Lane 2: Mouse IgG2a Kappa Isotype Control, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 140 kDa Non-reduced, 55 and 25 Reduced.

• Reactivity: Mu
• Applications: WB, ELISA, Flow, ICC/IF, PAGE
• Clone: M2AK
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Purified

Mouse IgG2a Kappa Isotype Control (M2AK) Summary

• Description: This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.
  
  Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Specificity: Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgA, IgM
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:1000-1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • SDS-Page
  • Western Blot
• Application Notes: This product has been tested in SDS-Page, ELISA, and western blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2a Kappa Isotype Control (NBP1-96981)(1)

Publication using NBP1-96981

• Dubner AM, Lu S, Jolly AJ et al. Smooth muscle-derived adventitial progenitor cells promote key cell type transitions controlling plaque stability in atherosclerosis in a Klf4-dependent manner bioRxiv 2023-07-23 [PMID: 37503181] (Flow Cytometry)