Rabbit anti-Human IgG2 Fc Secondary Antibody (EPR4418)

Product Discontinued

Rabbit anti-Human IgG2 Fc Secondary Antibody (EPR4418) Summary

• Immunogen: A synthetic peptide corresponding to residues in human IgG2 was used as an immunogen. This antibody does not cross-react with human IgG1, IgG3, and IgG4.
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500 - 1000
  • Western Blot 1:10000 - 20000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with: Human

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS 49%, Glycerol 50%, BSA 0.05%
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Background

IgG is a glycoprotein that is produced by plasma cells in response to an immunogen (1). It is the only class of immunoglobulins that can cross the placenta, which is mediated by a receptor on placental cells in the Fc region (1). Human IgG2 subclass has been identified to exist as an ensemble of distinct isoforms, designated IgG2-A, -B, and -A/B, which differ by the disulfide connectivity at the hinge region (2). IgG2-A is the known classic structure for the IgG2 subclass defined by structurally independent Fab domains and hinge region (3). IgG2-B is a structure defined by a symmetrical arrangement of a (C(H)1-C(L)-hinge)(2) complex with both Fab regions covalently linked to the hinge (3). IgG2-A/B represents an intermediate form, defined by an asymmetrical arrangement involving one Fab arm covalently linked to the hinge through disulfide bonds (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.