Mouse IgG1 Kappa Light Chain Isotype Control

Product Discontinued

Mouse IgG1 Kappa Light Chain Isotype Control Summary

• Description: This Mouse IgG1 Kappa immunoglobulin is useful as an isotype control for Mouse IgG1 Kappa antibody.
• Specificity: By immunoelectrophoresis the IgG1 was shown to 1) react with antiserum specific for mouse IgG1, 2) not react with antiserum specific for other immunoglobulins, and 3) produce a single precipitin arc with antiserum against mouse serum identical to that produced with anti-IgG1.
• Isotype: IgG1 Kappa
• Clonality: Polyclonal
• Host: Mouse
• Purity: Immunogen affinity purified

Applications/Dilutions

• Application Notes: Useful as a positive control in immunoassays. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS.
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Protein concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Background

Mouse IgG1 myeloma cells were grown in mice and ascitic fluids collected. The IgG1 was purified by a combination of salt fractionation, ion exchange and protein A chromatography. Protein concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis the IgG1 was shown to 1) react with antiserum specific for mouse IgG1, 2) not react with antiserum specific for other immunoglobulins, and 3) produce a single precipitation arc with antiserum against mouse serum identical to that produced with anti-IgG1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for Mouse IgG1, kappa L Chain Isotype Control (NBA1-21302)(4)

Publications using NBA1-21302

• Rubin RL, Hermanson TM, Bedrick EJ et al. Effect of cigarette smoke on autoimmunity in murine and human systemic lupus erythematosus. Toxicol Sci 2005-09-01 [PMID: 15947027]
• Laszik ZG, Zhou XJ, Ferrell GL et al. Down-regulation of endothelial expression of endothelial cell protein C receptor and thrombomodulin in coronary atherosclerosis. Am J Pathol 2001-09-01 [PMID: 11549570]
• Zhou XJ, Laszik Z, Wang XQ et al. Association of renal injury with increased oxygen free radical activity and altered nitric oxide metabolism in chronic experimental hemosiderosis. Lab Invest 2000-12-01 [PMID: 11140702]
• Fernando GJ, Stenzel DJ, Tindle RW et al. Peptide polymerisation facilitates incorporation into ISCOMs and increases antigen-specific IgG2a production. Vaccine 13 1460-1467. 1995-01-01 [PMID: 8578827]