Rat IgG1 Kappa Light Chain Isotype Control (KLH/G1-2-2)

ELISA: Rat IgG1 Kappa Light Chain Isotype Control (KLH/G1-2-2) [NBP1-51817] - FLISA plate was coated with Mouse Anti-Rat IgG1-UNLB (SB Cat. No. 3061-01), Mouse Anti-Rat IgG2a-UNLB (SB Cat. No. 3065-01), Mouse Anti-Rat IgG2b-UNLB (SB Cat. No. 3070-01), Mouse Anti-Rat IgG2c-UNLB (SB Cat. No. 3075-01), and Mouse Anti-Rat IgM-UNLB (SB Cat. No. 3080-01). Serially diluted Rat IgG1-PE (SB Cat. No. 0116-09) was captured and fluorescence intensity quantified.

• Reactivity: Rt
• Applications: ELISA, Flow, B/N, Ctrl
• Clone: KLH/G1-2-2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Low Endotoxin and Azide Free
• Concentration: Please contact technical services for concentration.

Rat IgG1 Kappa Light Chain Isotype Control (KLH/G1-2-2) Summary

• Description: This Rat IgG1 Kappa (KLH/G1-2-2) immunoglobulin is useful as an isotype control for Rat IgG1 Kappa antibody.
• Immunogen: KLH
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Rat

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Control
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
• Application Notes: Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50 mM Tris/1 mM MgCl2/50% glycerol, pH 8.0.
• Preservative: No Preservative
• Concentration: Please contact technical services for concentration.

Background

RTK2071 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.