Goat anti-Mouse IgG1 Heavy Chain Secondary Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Goat anti-Mouse IgG1 Heavy Chain Secondary Antibody Summary

Immunogen
Mouse IgG1
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity to mouse IgA, IgM, IgG2a, IgG2b, IgG2c, IgG3 and IgE is undetectable. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:1000- 1:30000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:1000 - 1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
Publications
Read Publications using
NB7508 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG1 Antibody (NB7508)(20)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ChIP, ICC/IF, WB.


Filter By Application
ChIP
(1)
ICC/IF
(2)
WB
(1)
All Applications
Filter By Species
Mouse
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB7508 Applications Species
Rodriguez-Morales O, Cabrera-Mata JJ, Carrillo-Sanchez SDC et al Electrolyzed Oxidizing Water Modulates the Immune Response in BALB/c Mice Experimentally Infected with Trypanosoma cruzi Pathogens Nov 26 2020 [PMID: 33238401] (ICC/IF, Mouse)

Details:
Citation using the HRP version of this antibody.
ICC/IF Mouse
Bigot P, Colli LM, Machiela MJ et al. Functional characterization of the 12p12 1 renal cancer-susceptibility locus implicates BHLHE41. Nat Commun. [PMID: 27384883] (WB)

Details:
Citation using the HRP form of this antibody.
WB
Wang T, Gilkes DM, Takano N et al. Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis. Proc Natl Acad Sci U S A. [PMID: 24938788] (ICC/IF)

Details:
Citation using the FITC form of this antibody.
ICC/IF
Chaturvedi P, Gilkes DM, Takano N, Semenza GL. Hypoxia-inducible factor-dependent signaling between triple-negative breast cancer cells and mesenchymal stem cells promotes macrophage recruitment. Proc Natl Acad Sci U S A. [PMID: 24799675] (ChIP)

Details:
Citation using the FITC form of this antibody.
ChIP
Lanckacker EA, Tournoy KG, Hammad H et al. Short cigarette smoke exposure facilitates sensitization and asthma development in mice. Eur Respir J. [PMID: 22903968]

Details:
Citation using the HRP form of this antibody.
Golden JW, Josleyn M, Mucker EM et al. Side-by-side comparison of gene-based smallpox vaccine with MVA in nonhuman primates. PLoS One. 2012 [PMID: 22860117]

Details:
Citation using the HRP form of this antibody.
Tawde SA, Chablani L, Akalkotkar A et al. Formulation and evaluation of oral microparticulate ovarian cancer vaccines. Vaccine. [PMID: 22750042]

Details:
Citation using the HRP form of this antibody.
Eisenbarth SC, Williams A, Colegio OR et al. NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells. Nature. [PMID: 22538615]

Details:
Citation using the HRP form of this antibody.
Yu L, Yamagishi J, Zhang S et al. Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice. Parasitol Int . [PMID: 22537971]

Details:
Citation using the HRP form of this antibody.
Oefner CM, Winkler A, Hess C et al. Tolerance induction with T cell-dependent protein antigens induces regulatory sialylated IgGs. J Allergy Clin Immunol. [PMID: 22502800]

Details:
Citation using the HRP form of this antibody.
Show All 20 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG1 Antibody (NB7508). (Showing 1 - 1 of 1 FAQs).

  1. I am wondering if you would be able to suggest does the antibody NB7508 mouse IgG1 react with the whole molecule of the IgG1 or just its heavy chain?
    • The immunogen for this antibody was whole IgG1 so since it is polyclonal, it is predicted to react with the whole molecule on multiple epitopes.

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