Mouse anti-Human IgG1 Fc fragment Secondary Antibody (NL 16)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr
Clone
NL 16
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Mouse anti-Human IgG1 Fc fragment Secondary Antibody (NL 16) Summary

Immunogen
Human IgG1 myeloma protein
Specificity
This antibody recognises an epitope within the CH2 domain of the Fc region of human IgG1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified

Applications/Dilutions

Application Notes
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4.
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Background

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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