Goat anti-Mouse IgG ImmPRESS™ Secondary Antibody [HRP Polymer]

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - onsil (double label): CD3 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector VIP substrate (purple); Ki67 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector SG substrate (blue/gray).

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Rhesus macaque formalin/paraffin lymph node: CD4 (1F6), ImmPRESS Anti-Mouse Ig Kit, ImmPACT DAB substrate (brown). Hematoxylin counterstain (blue). Courtesy of Charles Brown, NIH, NIAID, Laboratory of Molecular Microbiology.

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Tonsil: CD8 (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown). Hematoxylin QS counterstain (blue).

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Tonsil: Bcl-2 (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown). Hematoxylin QS counterstain (blue).

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Lymphoma: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPress anti-mouse Ig reagent, and Vector NovaRED peroxidase substrate ( red). Hematoxylin QS counterstain (blue)

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Tonsil: CD20 (m), ImmPRESS Anti-Mouse Ig Kit, ImmPACT VIP (purple) substrate

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPress anti-mouse Ig reagent, and Vector NovaRED peroxidase substrate (red). Hematoxylin QS counterstain

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Colon: PGP 9.5 (m, VP-P983), ImmPRESS Anti-Mouse Ig Kit, ImmPACT SG (blue-gray) substrate.

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Kidney: PGP 9.5 3, ImmPRESS Anti-Mouse Ig Kit, ImmPACT NovaRED (red) substrate. Hematoxylin (blue) counterstain.

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Colon: Peripherin (m, VP-P968), ImmPRESS Anti-Mouse Ig Kit, ImmPACT AEC (red) substrate

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Colon: PGP 9.5, ImmPRESS Anti-Mouse Ig Kit, ImmPACT SG (blue-gray) substrate.

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Colon: PGP 9.5 (m, VP-P983), ImmPRESS Anti-Mouse Ig Kit, ImmPACT AEC (red) substrate. Hematoxylin QS (blue) counterstain.

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Breast: Estrogen Receptor (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown). Hematoxylin counterstain (blue).

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40;), ImmPress anti-mouse Ig reagent, and Vector NovaRED peroxidase substrate (red). Hematoxylin QS counterstained

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Small Bowel: Peripherin (m), ImmPRESS Anti-Mouse Ig Kit, Vector NovaRED Substrate Kit (red). Hematoxylin QS Counterstain (blue).

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Small Bowel: CD10 (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown). Hematoxylin QS counterstain (blue).

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Colon: Peripherin, ImmPRESS Anti-Mouse Ig Kit, ImmPACT AEC (red) substrate.

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Tonsil: Cytokeratin (m), ImmPRESS Anti-Mouse Ig Kit, ImmPACT DAB (brown) substrate. Hematoxylin (blue) counterstain.

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Rhesus macaque formalin/paraffin lymph node: CD4 (1F6), ImmPRESS Anti-Mouse Ig Kit, ImmPACT DAB substrate (brown). Hematoxylin counterstain (blue). Courtesy of Charles Brown, NIH, NIAID, Laboratory of Molecular Microbiology.

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Lymphoma: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPress anti-mouse Ig reagent, and Vector NovaRED peroxidase substrate (red). Hematoxylin QS counterstain (blue)

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Breast: CD34 (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown). Hematoxylin counterstain (blue).

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Colon: Peripherin, ImmPRESS Anti-Mouse Ig Kit, ImmPACT NovaRED (red) substrate. Hematoxylin QS (blue) counterstain.

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Prostate: Smooth muscle actin (m), ImmPRESS Anti-Mouse Ig Kit, AEC Substrate Kit (red). Hematoxylin QS counterstain (blue). Mounted in VectaMount AQ Mounting Medium.

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Small Intestine (double label): ImmPRESS Anti-Mouse Ig using CD10 primary antibody with DAB/Ni (black) substrate, and ImmPRESS Anti-Mouse Ig using Cytokeratin 20 primary antibody with Vector NovaRED (red) substrate. No counterstain.

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Small Bowel (double label): Peripherin (m), ImmPRESS Anti-Mouse Ig Kit, Vector NovaRED (red) substrate. Desmin (m), ImmPRESS Anti-Mouse Ig Kit, DAB/Ni (black) substrate.

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Colon: Peripherin (m, VP-P968), ImmPRESS Anti-Mouse Ig Kit, ImmPACT SG (blue-gray) substrate.

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Tonsil (double label): CD3 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector NovaRED substrate (red); Ki67 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector DAB+Ni substrate (gray/black).

Immunohistochemistry: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Small Bowel: Cytokeratin 8/18 (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown), Hematoxylin QS (blue).

Immunofluorescence: Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Colon: anti-Cytokeratin AE1/AE3 (mouse MAb), ImmPRESS HRP Anti-Mouse IgG (made in goat), DAB Substrate Kit (brown).

Goat anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7452-NB] - Colon: anti-Cytokeratin AE1/AE3 (mouse MAb), ImmPRESS HRP Anti-Mouse IgG (made in goat), DAB Substrate Kit (brown).

• Reactivity: Mu
• Applications: ELISA, Func, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP Polymer

Goat anti-Mouse IgG ImmPRESS™ Secondary Antibody [HRP Polymer] Summary

• Description: Features:
• High sensitivity
• Low background
• One-step detection
• Micropolymer technology
• Enhanced accessibility to nuclear and membrane antigens
• Ready-to-use in a convenient dropper bottle
• Shorter assay time
• Simplified multiple labeling
• Immunogen: Mouse IgG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • In-situ Hybridization
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Preservative: No Preservative
• Purity: Affinity purified

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

The ImmPRESS(TM) polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.
The peroxidase micropolymers of the ImmPRESS(TM) HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.