Goat anti-Rat IgG ImmPRESS™ Secondary Antibody [HRP Polymer] (Mouse Adsorbed)

• Reactivity: Rt
• Applications: ELISA, Func, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP Polymer
• Format: Mouse Adsorbed

Goat anti-Rat IgG ImmPRESS™ Secondary Antibody [HRP Polymer] (Mouse Adsorbed) Summary

• Description: Features:
• High sensitivity
• Low background
• One-step detection
• Micropolymer technology
• Enhanced accessibility to nuclear and membrane antigens
• Ready-to-use in a convenient dropper bottle
• Shorter assay time
• Simplified multiple labeling
• Designed to detect rat primary antibodies on mouse tissues that may contain endogenous mouse immunoglobulins
• Immunogen: Rat IgG
• Specificity: Pre-adsorbed against mouse.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • In-situ Hybridization
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Preservative: No Preservative
• Purity: Affinity purified

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

The ImmPRESS(TM) polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.
The peroxidase micropolymers of the ImmPRESS(TM) HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG ImmPRESS(TM) Antibody (MP-7444-NB)(1)

Publication using MP-7444-NB

• Bessell E, Finlay RE, James LK, Ludewig B et Al. Stromal cell and B cell dialogue potentiates IL-33-enriched lymphoid niches to support eosinophil recruitment and function during type 2 immunity Cell Rep 2024-08-14 [PMID: 39141517]