Horse anti-Mouse IgG ImmPRESS™ Secondary Antibody [HRP Polymer] (Rat Adsorbed)

Immunohistochemistry: Horse anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7422-NB] - Rat Intestine: Desmin (m), ImmPRESS Anti-Mouse Ig Kit (Rat Adsorbed), DAB (brown) Substrate Kit. Hematoxylin QS (blue) counterstain.

Immunohistochemistry: Horse anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7422-NB] - Sections of rat spleen stained with a mouse antibody to Desmin and detected with a rat adsorbed or non-rat adsorbed ImmPRESS Anti-Mouse Ig Kit: ImmPRESS Anti-Mouse Ig Kit (Rat Adsorbed), top, ImmPRESS Anti-Mouse Ig (non-rat adsorbed), bottom; DAB (brown)

Immunohistochemistry: Horse anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7422-NB] - Rat Spleen: Desmin (m), ImmPRESS Anti-Mouse Ig Kit (Rat Adsorbed), DAB (brown) Substrate Kit. Hematoxylin QS (blue) counterstain.

Immunohistochemistry: Horse anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7422-NB] - No Primary negative controls of rat intestine stained with a rat adsorbed or non-rat adsorbed ImmPRESS Anti-Mouse Ig Kit. ImmPRESS Anti-Mouse Ig Kit (Rat Adsorbed), left, ImmPRESS Anti-Mouse Ig (non-rat adsorbed), right, DAB (brown) Substrate Kit.

Immunohistochemistry: Horse anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7422-NB] - Sections of rat intestine stained with a mouse antibody to Desmin and detected with a rat adsorbed or non-rat adsorbed ImmPRESS Anti-Mouse Ig Kit: ImmPRESS Anti-Mouse Ig Kit (Rat Adsorbed), left, ImmPRESS Anti-Mouse Ig (non-rat adsorbed), right; DAB (brown)

• Reactivity: Mu
• Applications: ELISA, Func, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Horse
• Conjugate: HRP Polymer
• Format: Rat Adsorbed

Horse anti-Mouse IgG ImmPRESS™ Secondary Antibody [HRP Polymer] (Rat Adsorbed) Summary

• Description: Features:
• High sensitivity
• Low background
• One-step detection
• Micropolymer technology
• Enhanced accessibility to nuclear and membrane antigens
• Ready-to-use in a convenient dropper bottle
• Shorter assay time
• Simplified multiple labeling
• Designed to detect mouse primary antibodies on rat tissues that may contain endogenous rat immunoglobulins
• Immunogen: Mouse IgG
• Specificity: Pre-adsorbed against rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Horse
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • In-situ Hybridization
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Preservative: No Preservative
• Purity: Affinity purified

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

The ImmPRESS(TM) polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.
The peroxidase micropolymers of the ImmPRESS(TM) HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.