Goat anti-Mouse IgG/IgM (H+L) Secondary Antibody [Texas Red] (Pre-adsorbed)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications FLISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Texas Red
Format
Pre-adsorbed
Concentration
LYOPH

Goat anti-Mouse IgG/IgM (H+L) Secondary Antibody [Texas Red] (Pre-adsorbed) Summary

Immunogen
Mouse IgG and IgM whole molecules
Specificity
No reaction was observed against bovine, horse or human serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Multi-step

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
Application Notes
This secondary antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water (or equivalent).

Notes

This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse IgM

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Mouse IgG/IgM, H/L Chains Antibody (NBP1-73373) (0)

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Other Available Formats

Alkaline Phosphatase NBP1-72626
Allophycocyanin/Cy7 NBP1-72701APCCY7
Biotin NBP1-73372
DyLight 405 NBP1-72701V
DyLight 488 NBP1-72701G
DyLight 550 NBP1-72701R
DyLight 594 NBP1-72701DL594
DyLight 650 NBP1-72701C
DyLight 680 NBP1-72701FR
DyLight 755 NBP1-72701IR
FITC NBP1-73371
HRP NBP1-73567
Janelia Fluor 549 NBP1-72701JF549
Janelia Fluor 646 NBP1-72701JF646
PE/Atto594 NBP1-72701PEATT594
PE/Cy5.5 NBP1-72701PECY55
PE/Cy7 NBP1-72701PECY7
Rhodamine NBP1-73370
Texas Red NBP1-73373

Additional IgG/IgM (H+L) Products

Array NBP1-73373

Blogs on IgG/IgM (H+L)

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