Donkey anti-Human IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Donkey
Conjugate
Unconjugated
Format
Pre-adsorbed
Concentration
1 mg/ml

Order Details

Donkey anti-Human IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed) Summary

Immunogen
Human IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Frozen 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
Application Notes
This product is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IgG (H+L) Antibody (NBP2-60667) (0)

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Other Available Formats

Alkaline Phosphatase NBP1-72668
Allophycocyanin/Cy7 NBP2-60667APCCY7
Biotin NBP1-73618
DyLight 350 NBP2-60667UV
DyLight 405 NBP2-60667V
DyLight 488 NBP2-60667G
DyLight 550 NBP2-60667R
DyLight 594 NBP2-60667DL594
DyLight 650 NBP2-60667C
DyLight 680 NBP2-60667FR
DyLight 755 NBP2-60667IR
FITC NBP1-73617
HRP NBP1-73738
Janelia Fluor 549 NBP2-60667JF549
Janelia Fluor 646 NBP2-60667JF646
PE/Atto594 NBP2-60667PEATT594
PE/Cy5.5 NBP2-60667PECY55
PE/Cy7 NBP2-60667PECY7
Rhodamine NBP1-73616
Texas Red NBP1-73619

Additional IgG (H+L) Products

Array NBP2-60667

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