Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP]


Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H]
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H]
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H] - Analysis of anti-Caspase 1, using Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] at 1:5,000.
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP][NBP2-30348H]

Product Details

Reactivity RbSpecies Glossary
Applications WB, ELISA

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Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] Summary

Rabbit Ig (H + L)
HRP Goat Anti-Rabbit IgG is reported to have minimal crossreactivity with human, mouse or rat serum proteins
Immunogen affinity purified


  • Western Blot 1:1000-1:5000
  • ELISA 1:1000-1:20000
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Reviewed Applications
Read 1 Review rated 5
NBP2-30348H in the following applications:

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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Aqueous buffered solution containing BSA
ProClin 150
Immunogen affinity purified


Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).


1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP2-30348H)(13)

We have publications tested in 2 applications: ELISA, WB.

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Chen Tc, Liu Hw, Russell A et Al. Large increases in plasma fast skeletal muscle troponin I after whole-body eccentric exercises J Sci Med Sport Feb 5 2020 [PMID: 32067915] (ELISA) ELISA
Steimle BL, Smith FM, Kosman DJ. The solute carriers ZIP8 and ZIP14 regulate manganese accumulation in brain microvascular endothelial cells and control brain manganese levels J Biol Chem Nov 9 2019 [PMID: 31699897] (WB) WB
Olivas-Aguirre M, Torres-Lopez L, Valle-Reyes JS et al. Cannabidiol directly targets mitochondria and disturbs calcium homeostasis in acute lymphoblastic leukemia Cell Death Dis Oct 16 2019 [PMID: 31611561] (WB) WB
Torres-Lopez L, Maycotte P, Linan-Rico A et al. Tamoxifen induces toxicity, causes autophagy, and partially reverses dexamethasone resistance in Jurkat T cells J Leukoc Biol Jan 16 2019 [PMID: 30645008] (WB) WB
Liu F, Wang W, Li S et al. MicroRNA 141 represses nasopharyngeal carcinoma growth through inhibiting BMI1 Oncol Lett Nov 9 2018 [PMID: 30405786] (WB) WB
Cai JY, Xu TT, Wang Y et al. Histone deacetylase HDAC4 promotes the proliferation and invasion of glioma cells Int J Oncol Oct 2 2018 [PMID: 30272277]
Tarade D, Ma D, Pignanelli C et al. Structurally simplified biphenyl combretastatin A4 derivatives retain in vitro anti-cancer activity dependent on mitotic arrest PLoS One Mar 3 2017 [PMID: 28253265] (WB) WB
Ma D, Pignanelli C, Tarade D et al. Cancer Cell Mitochondria Targeting by Pancratistatin Analogs is Dependent on Functional Complex II and III. Sci Rep 2017 Feb 21 [PMID: 28220885] (WB) WB
Song YH, Warncke C, Choi SJ et al. Breast cancer-derived extracellular vesicles stimulate myofibroblast differentiation and pro-angiogenic behavior of adipose stem cells Matrix Biol Dec 4 2016 [PMID: 27913195] (WB) WB
Martin C, Espaillat MP, Santiago-Schwarz F. IL-10 restricts dendritic cell (DC) growth at the monocyte-to-monocyte-derived DC interface by disrupting anti-apoptotic and cytoprotective autophagic molecular machinery. Immunol. Res. 2015 Sep 22 [PMID: 26395023] (WB) WB
Show All 13 Publications.

Review for IgG (H+L) Antibody (NBP2-30348H) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-30348H:
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Images Ratings Applications Species Date Details
Western Blot IgG (H+L) NBP2-30348H
reviewed by:
Arun Satelli
WB Human 10/16/2014


ApplicationWestern Blot
Sample TestedHuman Cancer cell lysates
CommentsThough suggested dilutions are 1:1000 to 1:5000, 1:1000 is the one that works perfectly.

Primary Anitbody

Dilution Ratio1:1000


CommentsThough suggested dilutions are 1:1000 to 1:5000, 1:1000 is the one that works perfectly.

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HRP NBP2-30348H

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Array NBP2-30348H

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Arun Satelli
Application: WB
Species: Human