Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP]

Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H]

Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H]

Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H] - Analysis of anti-Caspase 1, using Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] at 1:5,000.

Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP][NBP2-30348H]

Product Discontinued

Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] Summary

• Immunogen: Rabbit Ig (H + L)
• Specificity: HRP Goat Anti-Rabbit IgG is reported to have minimal crossreactivity with human, mouse or rat serum proteins
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:20000
  • Western Blot 1:1000-1:5000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Use in Human reported in scientific literature (PMID:33421634)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Aqueous buffered solution containing BSA
• Preservative: ProClin 150
• Purity: Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP2-30348H)(20)

Publications using NBP2-30348H

• Nation CS,Stephany-Brassesco I,Kelly BL et al' Transgenic overexpression of heat shock protein (HSP83) enhances protein kinase A activity, disrupts GP63 surface protease expression and alters promastigote morphology in Leishmania amazonensis Molecular and biochemical parasitology 2023-05-05 [PMID: 37150327] (Western Blot)
• Schwarz N, Fernando S, Chen YC et al. Colchicine exerts anti-atherosclerotic and -plaque-stabilizing effects targeting foam cell formation FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023-04-01 [PMID: 36856983]
• Steimle BL, Bailey DK, Smith FM Et al. Calcium and the Ca-ATPase SPCA1 modulate plasma membrane abundance of ZIP8 and ZIP14 to regulate Mn(II) uptake in brain microvascular endothelial cells J Biol Chem 2022-07-05 [PMID: 35787370] (WB)
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Marino M, Zhou L, Rincon MY Et al. AAV-mediated delivery of an anti-BACE1 VHH alleviates pathology in an Alzheimer's disease model EMBO Mol Med 2022-03-30 [PMID: 35352880] (WB)
  
  Details:
  Citation using the HRP version of this antibody.
• Bailey Dk, Clark W, Kosman Dj The iron chelator, PBT434, modulates transcellular iron trafficking in brain microvascular endothelial cells PloS one 2021-07-26 [PMID: 34310628] (WB)
• Steimle BL, Smith FM, Kosman DJ. The solute carriers ZIP8 and ZIP14 regulate manganese accumulation in brain microvascular endothelial cells and control brain manganese levels J Biol Chem 2019-11-09 [PMID: 31699897] (WB)
• Liu Y, Chen R, Li L et al. The triterpenoids-enriched extracts from Antrodia cinnamomea mycelia attenuate alcohol-induced chronic liver injury via suppression lipid accumulation in C57BL/6 mice Food Science and Human Wellness 2021-07-01
• Ghodsian N, Yeandle A, Gieseg SP Foam cell formation but not oxLDL cytotoxicity is inhibited by CD36 down regulation by the macrophage antioxidant 7,8-dihydroneopterin The international journal of biochemistry & cell biology Jan 6 2021 [PMID: 33421634] (WB, Human)
• Chen Tc, Liu Hw, Russell A et Al. Large increases in plasma fast skeletal muscle troponin I after whole-body eccentric exercises J Sci Med Sport 2020-02-05 [PMID: 32067915] (ELISA)
• Olivas-Aguirre M, Torres-Lopez L, Valle-Reyes JS et al. Cannabidiol directly targets mitochondria and disturbs calcium homeostasis in acute lymphoblastic leukemia Cell Death Dis 2019-10-16 [PMID: 31611561] (WB)
• Torres-Lopez L, Maycotte P, Linan-Rico A et al. Tamoxifen induces toxicity, causes autophagy, and partially reverses dexamethasone resistance in Jurkat T cells J Leukoc Biol 2019-01-16 [PMID: 30645008] (WB)
• Liu F, Wang W, Li S et al. MicroRNA 141 represses nasopharyngeal carcinoma growth through inhibiting BMI1 Oncol Lett 2018-11-09 [PMID: 30405786] (WB)
• Cai JY, Xu TT, Wang Y et al. Histone deacetylase HDAC4 promotes the proliferation and invasion of glioma cells Int J Oncol 2018-10-02 [PMID: 30272277]
• Tarade D, Ma D, Pignanelli C et al. Structurally simplified biphenyl combretastatin A4 derivatives retain in vitro anti-cancer activity dependent on mitotic arrest PLoS One 2017-03-03 [PMID: 28253265] (WB)
• Ma D, Pignanelli C, Tarade D et al. Cancer Cell Mitochondria Targeting by Pancratistatin Analogs is Dependent on Functional Complex II and III. Sci Rep 2017-02-21 [PMID: 28220885] (WB)
• Song YH, Warncke C, Choi SJ et al. Breast cancer-derived extracellular vesicles stimulate myofibroblast differentiation and pro-angiogenic behavior of adipose stem cells Matrix Biol 2016-12-04 [PMID: 27913195] (WB)
• Martin C, Espaillat MP, Santiago-Schwarz F. IL-10 restricts dendritic cell (DC) growth at the monocyte-to-monocyte-derived DC interface by disrupting anti-apoptotic and cytoprotective autophagic molecular machinery. Immunol. Res. 2015-09-22 [PMID: 26395023] (WB)
• Patil RH, Babu RL, Naveen Kumar M et al. Anti-Inflammatory Effect of Apigenin on LPS-Induced Pro-Inflammatory Mediators and AP-1 Factors in Human Lung Epithelial Cells. Inflammation. 2015-08-16 [PMID: 26276128] (WB)
• Pang X, Wang Z, Yin D, Zhang Z. Overexpression of hypoxia-inducible factor prolyl- hydoxylase attenuated by HCG-induced vascular endothelial growth factor expression in luteal cells. Mol Med Rep 2015-05-16 [PMID: 25975603]
• Patil RH, Babu RL, Naveen Kumar M et al. Apigenin inhibits PMA-induced expression of pro-inflammatory cytokines and AP-1 factors in A549 cells Mol. Cell. Biochem. 2015-02-10 [PMID: 25666088] (WB)

Review for IgG (H+L) Antibody (NBP2-30348H) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Arun Satelli WB Human 10/16/2014

Summary

• Application: Western Blot
• Sample Tested: Human Cancer cell lysates
• Species: Human