Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP]

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Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H]
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H]
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H] - Analysis of anti-Caspase 1, using Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] at 1:5,000.
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP2-30348H] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP][NBP2-30348H]

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
HRP

Order Details

Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
Rabbit Ig (H + L)
Specificity
HRP Goat Anti-Rabbit IgG is reported to have minimal crossreactivity with human, mouse or rat serum proteins
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:1000-1:20000
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NBP2-30348H in the following applications:

Publications
Read Publications using NBP2-30348H.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Aqueous buffered solution containing BSA
Preservative
ProClin 150
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP2-30348H)(13)

We have publications tested in 2 applications: ELISA, WB.


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(1)
WB
(10)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP2-30348H Applications Species
Chen Tc, Liu Hw, Russell A et Al. Large increases in plasma fast skeletal muscle troponin I after whole-body eccentric exercises J Sci Med Sport Feb 5 2020 [PMID: 32067915] (ELISA) ELISA
Steimle BL, Smith FM, Kosman DJ. The solute carriers ZIP8 and ZIP14 regulate manganese accumulation in brain microvascular endothelial cells and control brain manganese levels J Biol Chem Nov 9 2019 [PMID: 31699897] (WB) WB
Olivas-Aguirre M, Torres-Lopez L, Valle-Reyes JS et al. Cannabidiol directly targets mitochondria and disturbs calcium homeostasis in acute lymphoblastic leukemia Cell Death Dis Oct 16 2019 [PMID: 31611561] (WB) WB
Torres-Lopez L, Maycotte P, Linan-Rico A et al. Tamoxifen induces toxicity, causes autophagy, and partially reverses dexamethasone resistance in Jurkat T cells J Leukoc Biol Jan 16 2019 [PMID: 30645008] (WB) WB
Liu F, Wang W, Li S et al. MicroRNA 141 represses nasopharyngeal carcinoma growth through inhibiting BMI1 Oncol Lett Nov 9 2018 [PMID: 30405786] (WB) WB
Cai JY, Xu TT, Wang Y et al. Histone deacetylase HDAC4 promotes the proliferation and invasion of glioma cells Int J Oncol Oct 2 2018 [PMID: 30272277]
Tarade D, Ma D, Pignanelli C et al. Structurally simplified biphenyl combretastatin A4 derivatives retain in vitro anti-cancer activity dependent on mitotic arrest PLoS One Mar 3 2017 [PMID: 28253265] (WB) WB
Ma D, Pignanelli C, Tarade D et al. Cancer Cell Mitochondria Targeting by Pancratistatin Analogs is Dependent on Functional Complex II and III. Sci Rep 2017 Feb 21 [PMID: 28220885] (WB) WB
Song YH, Warncke C, Choi SJ et al. Breast cancer-derived extracellular vesicles stimulate myofibroblast differentiation and pro-angiogenic behavior of adipose stem cells Matrix Biol Dec 4 2016 [PMID: 27913195] (WB) WB
Martin C, Espaillat MP, Santiago-Schwarz F. IL-10 restricts dendritic cell (DC) growth at the monocyte-to-monocyte-derived DC interface by disrupting anti-apoptotic and cytoprotective autophagic molecular machinery. Immunol. Res. 2015 Sep 22 [PMID: 26395023] (WB) WB
Show All 13 Publications.

Review for IgG (H+L) Antibody (NBP2-30348H) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-30348H:
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WB
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Images Ratings Applications Species Date Details
Western Blot IgG (H+L) NBP2-30348H
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5
reviewed by:
Arun Satelli
WB Human 10/16/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman Cancer cell lysates
SpeciesHuman
CommentsThough suggested dilutions are 1:1000 to 1:5000, 1:1000 is the one that works perfectly.

Primary Anitbody

Dilution Ratio1:1000

Comments

CommentsThough suggested dilutions are 1:1000 to 1:5000, 1:1000 is the one that works perfectly.

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FAQs for IgG (H+L) Antibody (NBP2-30348H) (0)

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Other Available Formats

HRP NBP2-30348H

Additional IgG (H+L) Products

Array NBP2-30348H

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Recent Reviews

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Arun Satelli
10/16/2014
Application: WB
Species: Human