Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).
The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).
3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268
4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
Publications for IgG (H+L) Antibody (NBP2-30347H)(14)
We have publications tested in 2 applications: Dir ELISA, WB.
Luo Z, Liu Z, Yu H et al. Multifunctional mesoporous polydopamine near-infrared photothermal controlled release of kartogenin for cartilage repair Materials & Design 2023-07-01
Chu Q, Dou B, Wang S et al. Formula Improves Mouse Hematopoietic Function after Cyclophosphamide-Induced Damage via Enhancing Macrophage Colony-Stimulating Factor Concentrations Research Square 2020-06-12
Liu Y, Chen R, Li L et al. The triterpenoids-enriched extracts from Antrodia cinnamomea mycelia attenuate alcohol-induced chronic liver injury via suppression lipid accumulation in C57BL/6 mice Food Science and Human Wellness 2021-07-01
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![Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NBP2-30347H] - Analysis of anti-TRF2 antibody (cat # IMG-124) using Imgenex's Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] (cat # 20101) at 1:5000.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NBP2-30347H-img0003.jpg "Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NBP2-30347H] - Analysis of anti-TRF2 antibody (cat # IMG-124) using Imgenex's Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] (cat # 20101) at 1:5000.")
