Goat anti-Canine IgG (H+L) Secondary Antibody [FITC]

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Immunofluorescence: Goat anti-Canine IgG (H+L) Secondary Antibody [FITC] [NBP1-75720] - Rat liver stained with anti-nuclear antibody followed by goat anti-canine IgG FITC conjugated secondary antibody. Image from ...read more

Product Details

Summary
Reactivity CaSpecies Glossary
Applications WB, ELISA, IA, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
FITC
Concentration
1.5 mg/ml

Order Details

Goat anti-Canine IgG (H+L) Secondary Antibody [FITC] Summary

Immunogen
Purified Dog IgG (H&L)
Specificity
Based on IEP, this antibody reacts with heavy chains on dog IgG and light chains on all dog immunoglobulins
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:20000
  • ELISA 1:2000 - 1:20000
  • Immunohistochemistry 1:500 - 1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-75720 in the following applications:

Reactivity Notes

Canine IgG, H/L Chains

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
1.5 mg/ml
Purity
>95%, by SDS-PAGE

Notes

Affinity purified antibody is > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. Fluorescein-5-Isothiocyanate (FITC) Ex = 494 nm; Em = 518 nm

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Review for IgG (H+L) Antibody (NBP1-75720) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-75720:
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IF
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence IgG (H+L) NBP1-75720
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reviewed by:
A G
IF Other 03/10/2016
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Summary

ApplicationImmunofluorescence
Sample TestedDog serum on Rat liver slide
SpeciesOther
Lot34-93-101613

Blocking

Blocking DetailsNA

Primary Anitbody

Dilution RatioNA

Secondary Antibody

Secondary DescriptionGoat anti dog IgG H+L
Secondary Manufacturer Cat#NBP1-75720
Secondary Concentration1ug/ml

Details

Detection NotesFluorescent microscope
Fixation DetailsSample was diluted and different dilutions were tested.
Wash DescriptionPBS in couplin jar for 5 mins

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-75720) (0)

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Array NBP1-75720

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A G
03/10/2016
Application: IF
Species: Other