Donkey anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 594] (Pre-absorbed)

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Immunocytochemistry/ Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 594] [NBP1-75637] - West Nile virus infection of mosquito cells were stained with primary antibody followed by DyLight ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications Flow, ICC/IF, IM
Clonality
Polyclonal
Host
Donkey
Conjugate
DyLight 594
Format
Pre-absorbed
Concentration
LYOPH

Donkey anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 594] (Pre-absorbed) Summary

Immunogen
Purified Rabbit IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with mouse IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20 - 1:2000
  • Immunomicroscopy 1:20 - 1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-75637 in the following applications:

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with sterilized deionized water. Centrifuge to remove any particulates.

Notes

Affinity purified antibody is greater than 95% based on SDS-PAGE. Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. DyLight 594 (Ex = 593 nm; Em = 618 nm). After reconstituted, prepare fresh working dilution daily.

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Review for IgG (H+L) Antibody (NBP1-75637) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: IF.

Reviews using NBP1-75637:
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Images Ratings Applications Species Date Details
Immunofluorescence IgG (H+L) NBP1-75637
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reviewed by:
Tonya Colpitts
IF Other 10/20/2014
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Summary

ApplicationImmunofluorescence
Sample TestedMosquito cell
SpeciesOther
CommentsMake sure you wash well, this antibody is potent

Blocking

Blocking Details1% BSA for 20 minutes at RT

Primary Anitbody

Dilution RatioAntibody against West Nile virus envelope protein made in our lab, diluted 1/500 in 1% BSA, incubated 20 mins at RT

Secondary Antibody

Secondary Descriptionthis is the secondary antibody
Secondary ConcentrationUsed at 1/1000 in 1% BSA

Details

Fixation Detailsfixation in paraformaldehde (4%) for 30 mins at RT
Wash Descriptionwashed 3 times with PBS

Comments

CommentsMake sure you wash well, this antibody is potent

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-75637) (0)

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Tonya Colpitts
10/20/2014
Application: IF
Species: Other