Chicken anti-Mouse IgG (H+L) Secondary Antibody [DyLight 594]

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Immunocytochemistry/ Immunofluorescence: Chicken anti-Mouse IgG (H+L) Secondary Antibody [DyLight 594] [NBP1-75555] - analysis of Laminin in Vero cells. Cells were fixed in 4% formaldehyde for 20 minutes at room ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IM
Clonality
Polyclonal
Host
Chicken
Conjugate
DyLight 594
Concentration
LYOPH

Chicken anti-Mouse IgG (H+L) Secondary Antibody [DyLight 594] Summary

Immunogen
Purified Mouse IgG (H&L)
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20 - 1:2000
  • Immunomicroscopy 1:20 - 1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-75555 in the following applications:

Reactivity Notes

Mouse IgG, H/L Chains

Packaging, Storage & Formulations

Storage
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with sterilized deionized water. Centrifuge to remove any particulates.

Notes

Affinity purified antibody is > 95% based on SDS-PAGE. Chicken Ig fraction was prepared using eggs from healthy hens of US origin. DyLight 594 (Ex = 593 nm; Em = 618 nm). After reconstituted, prepare fresh working dilution daily.

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Mouse IgG, H/L Chains Antibody (NBP1-75555) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: IF.

Reviews using NBP1-75555:
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Images Ratings Applications Species Date Details
Immunofluorescence IgG (H+L) NBP1-75555
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reviewed by:
Anonymous
IF Other 11/12/2014
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Summary

ApplicationImmunofluorescence
Sample TestedVero cell
SpeciesOther

Blocking

Blocking Detailsblocked in 1% BSA in PBS, 20 mins at RT

Primary Anitbody

Dilution Ratioprimary antibody against laminin, diluted 1/500 in 1%BSA, incubated 20 min RT

Secondary Antibody

Secondary Descriptionthis is the secondary

Details

Detection Notes40X and 600ms
Fixation Detailsfixed in 4% paraformaldehyde, 20 mins at RT
Wash Descriptionwashed three times between each incubation with PBS

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Mouse IgG, H/L Chains Antibody (NBP1-75555) (0)

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Recent Reviews

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Anonymous
11/12/2014
Application: IF
Species: Other