Chicken anti-Goat IgG (H+L) Secondary Antibody [DyLight 800] (Pre-adsorbed)

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Product Details

Summary
Reactivity GtSpecies Glossary
Applications Flow, IM
Clonality
Polyclonal
Host
Chicken
Conjugate
DyLight 800
Format
Pre-adsorbed
Concentration
LYOPH

Chicken anti-Goat IgG (H+L) Secondary Antibody [DyLight 800] (Pre-adsorbed) Summary

Immunogen
Purified Goat IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with and heavy (gamma) chains on goat IgG and light chains on all goat immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human, mouse, rabbit IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20 - 1:2000
  • Immunomicroscopy 1:20 - 1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.

Reactivity Notes

Based on IEP, this antibody reacts with and heavy (gamma) chains on goat IgG and light chains on all goat immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Reconstitute with sterilized deionized water. Centrifuge to remove any particulates.

Notes

Affinity purified antibody is > 95% based on SDS-PAGE. Chicken Ig fraction was prepared using eggs from healthy hens of US origin. DyLight 800 (Ex = 777 nm; Em = 794 nm). After reconstituted, prepare fresh working dilution daily.

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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