Donkey anti-Sheep IgG (H+L) Secondary Antibody [Janelia Fluor 646] (Pre-adsorbed) Summary
Purified Sheep IgG (H&L)
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on sheep IgG and light chains on all sheep immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human, mouse, rabbit IgG serum proteins.
>95%, by SDS-PAGE
- Western Blot 1:100-1:2000
- ELISA 1:100-1:2000
- Flow Cytometry 1:10-1:1000
- Immunocytochemistry/Immunofluorescence 1:10-1:500
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:10-1:500
- Immunohistochemistry-Paraffin 1:10-1:500
Optimal dilution of this antibody should be experimentally determined.
Based on IEP, this antibody reacts with heavy (gamma) chains on sheep IgG and light chains on all sheep immunoglobulins.
Packaging, Storage & Formulations
Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
>95%, by SDS-PAGE
Purity > 95% based on SDS-PAGE. Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed
for 1 year from date of receipt.
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