Donkey anti-Sheep IgG (H+L) Secondary Antibody [Janelia Fluor 646] (Pre-adsorbed)

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Product Details

Summary
Reactivity ShSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Donkey
Conjugate
Janelia Fluor 646
Format
Pre-adsorbed

Order Details

Donkey anti-Sheep IgG (H+L) Secondary Antibody [Janelia Fluor 646] (Pre-adsorbed) Summary

Immunogen
Purified Sheep IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on sheep IgG and light chains on all sheep immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human, mouse, rabbit IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on sheep IgG and light chains on all sheep immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
>95%, by SDS-PAGE

Notes

Purity > 95% based on SDS-PAGE. Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-75440JF646) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Alkaline Phosphatase NBP1-75441
Allophycocyanin/Cy7 NBP1-75440APCCY7
Biotin NBP1-75442
DyLight 350 NBP1-75676
DyLight 405 NBP1-75440V
DyLight 488 NBP1-75446
DyLight 550 NBP1-75440R
DyLight 594 NBP1-75678
DyLight 633 NBP1-75679
DyLight 650 NBP1-75440C
DyLight 680 NBP1-75440FR
DyLight 755 NBP1-75440IR
FITC NBP1-75443
HRP NBP1-75444
Janelia Fluor 549 NBP1-75440JF549
Janelia Fluor 646 NBP1-75440JF646
PE/Atto594 NBP1-75440PEATT594
PE/Cy5.5 NBP1-75440PECY55
PE/Cy7 NBP1-75440PECY7

Additional IgG (H+L) Products

Array NBP1-75440JF646

Blogs on IgG (H+L)

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Recombinant Monoclonal Antibodies

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