Donkey anti-Sheep IgG (H+L) Secondary Antibody [PE/Cy7]

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Product Details

Summary
Reactivity ShSpecies Glossary
Applications WB, CLIA, ELISA, Flow, IA, ICC/IF, IHC, IM
Clonality
Polyclonal
Host
Donkey
Conjugate
PE/Cy7

Order Details

Donkey anti-Sheep IgG (H+L) Secondary Antibody [PE/Cy7] Summary

Immunogen
This Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against purified sheep IgG (H&L).
Specificity
Based on IEP, this Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) heavy gamma chains on sheep IgG and light chains on all sheep immunoglobulins. This antibody has been pre-adsorbed against human or rabbit IgG
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on sheep IgG and light chains on all sheep immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
>95%, by SDS-PAGE

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP1-75433PECY7) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-75433PECY7) (0)

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Other Available Formats

Alkaline Phosphatase NBP1-75434
Allophycocyanin/Cy7 NBP1-75433APCCY7
Biotin NBP1-75435
DyLight 350 NBP1-75433UV
DyLight 405 NBP1-75433V
DyLight 488 NBP1-75439
DyLight 550 NBP1-75672
DyLight 594 NBP1-75673
DyLight 650 NBP1-75675
DyLight 680 NBP1-75433FR
DyLight 755 NBP1-75433IR
FITC NBP1-75436
HRP NBP1-75437
Janelia Fluor 549 NBP1-75433JF549
Janelia Fluor 646 NBP1-75433JF646
PE/Atto594 NBP1-75433PEATT594
PE/Cy5.5 NBP1-75433PECY55
PE/Cy7 NBP1-75433PECY7

Additional IgG (H+L) Products

Array NBP1-75433PECY7

Research Areas for IgG (H+L) Antibody (NBP1-75433PECY7)

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