Goat F(ab')2 anti-Rat IgG (H+L) Secondary Antibody [Janelia Fluor 549] (Pre-adsorbed)

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Janelia Fluor 549
Format
Pre-adsorbed

Goat F(ab')2 anti-Rat IgG (H+L) Secondary Antibody [Janelia Fluor 549] (Pre-adsorbed) Summary

Immunogen
Purified Rat IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human, mouse IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
>90%, by SDS-PAGE

Notes

Purity > 90% based on SDS-PAGE Small amounts of intact IgG may be present.. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-75405JF549) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Allophycocyanin/Cy7 NBP1-75405APCCY7
Biotin NBP1-75407
DyLight 350 NBP1-75405UV
DyLight 405 NBP1-75405V
DyLight 488 NBP1-75411
DyLight 550 NBP1-76110
DyLight 594 NBP1-75405DL594
DyLight 650 NBP1-75405C
DyLight 680 NBP1-75405FR
DyLight 755 NBP1-75405IR
FITC NBP1-75408
HRP NBP1-75409
Janelia Fluor 549 NBP1-75405JF549
Janelia Fluor 646 NBP1-75405JF646
PE/Atto594 NBP1-75405PEATT594
PE/Cy5.5 NBP1-75405PECY55
PE/Cy7 NBP1-75405PECY7

Additional IgG (H+L) Products

Array NBP1-75405JF549

Blogs on IgG (H+L)

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Recombinant Monoclonal Antibodies

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