Chicken anti-Rat IgG (H+L) Secondary Antibody [DyLight 488]


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Product Details

Reactivity RtSpecies Glossary
Applications WB, CLIA, ELISA, Flow, IA, ICC/IF, IHC, IM
DyLight 488

Chicken anti-Rat IgG (H+L) Secondary Antibody [DyLight 488] Summary

This Chicken anti-Rat IgG (H+L) Secondary Antibody was developed against purified rat IgG (H&L).
Based on IEP, this Chicken anti-Rat IgG (H+L) Secondary Antibody reacts with heavy gamma chains on rat IgG and light chains on all rat immunoglobulins.
>95%, by SDS-PAGE


  • Western Blot : 1:100-1:2000
  • ELISA : 1:100-1:2000
  • Flow Cytometry : 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence : 1:10 - 1:500
  • Immunohistochemistry : 1:10 - 1:500
  • Immunomicroscopy : 1:10 - 1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
>95%, by SDS-PAGE


Chicken products cannot be exported to Canada.

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.


Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).


1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Other Available Formats

Allophycocyanin/Cy7 NBP1-75356APCCY7
DyLight 350 NBP1-75356UV
DyLight 405 NBP1-75356V
DyLight 488 NBP1-75356G
DyLight 550 NBP1-75356R
DyLight 594 NBP1-75356DL594
DyLight 650 NBP1-75356C
DyLight 680 NBP1-75356FR
DyLight 755 NBP1-75356IR
HRP NBP1-75360
Janelia Fluor 549 NBP1-75356JF549
Janelia Fluor 646 NBP1-75356JF646
PE/Atto594 NBP1-75356PEATT594
PE/Cy5.5 NBP1-75356PECY55
PE/Cy7 NBP1-75356PECY7

Additional IgG (H+L) Products

Array NBP1-75356G

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