Chicken anti-Rabbit IgG (H+L) Secondary Antibody [TRITC] (Pre-adsorbed)

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Immunocytochemistry/ Immunofluorescence: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [TRITC] [NBP1-75270]

Product Details

Summary
Reactivity RbSpecies Glossary
Applications ELISA, Flow, ICC/IF, IM
Clonality
Polyclonal
Host
Chicken
Conjugate
TRITC
Format
Pre-adsorbed
Concentration
LYOPH

Order Details

Chicken anti-Rabbit IgG (H+L) Secondary Antibody [TRITC] (Pre-adsorbed) Summary

Immunogen
Purified Rabbit IgG (H&L)
Specificity
Based on IEP, this antibody reacts with:
heavy (g) chains on rabbit IgG
light chains on all rabbit immunoglobulins
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20-1:2000
  • Immunomicroscopy 1:20-1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.

Reactivity Notes

Based on IEP, no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
IgG from human or mouse

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Notes

Purity > 95% based on SDS-PAGE. Ig fraction was prepared using eggs obtained from healthy hens of US origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Array NBP1-75270

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