Goat anti-Mouse IgG (H+L) Secondary Antibody [DyLight 405]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IA, ID, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
DyLight 405

Goat anti-Mouse IgG (H+L) Secondary Antibody [DyLight 405] Summary

Immunogen
This Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against purified mouse IgG (H&L).
Specificity
Based on IEP, this Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody has been pre-adsorbed against human IgG or serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Western Blot : 1:100-1:2000
  • ELISA : 1:100-1:2000
  • Immunohistochemistry : 1:10 - 1:500
  • Immunoprecipitation : 1:10 - 1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
>90%, by SDS-PAGE

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Other Available Formats

Alkaline Phosphatase NBP1-75169
Allophycocyanin/Cy7 NBP1-75168APCCY7
Biotin NBP1-75170
DyLight 350 NBP1-75168UV
DyLight 405 NBP1-75168V
DyLight 488 NBP1-75168G
DyLight 550 NBP1-75168R
DyLight 594 NBP1-75168DL594
DyLight 650 NBP1-75168C
DyLight 680 NBP1-75168FR
DyLight 755 NBP1-75168IR
FITC NBP1-75171
HRP NBP1-75172
Janelia Fluor 549 NBP1-75168JF549
Janelia Fluor 646 NBP1-75168JF646
PE/Atto594 NBP1-75168PEATT594
PE/Cy5.5 NBP1-75168PECY55
PE/Cy7 NBP1-75168PECY7

Additional IgG (H+L) Products

Array NBP1-75168V

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