Chicken anti-Mouse IgG (H+L) Secondary Antibody [Alkaline Phosphatase]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Chicken
Conjugate
Alkaline Phosphatase

Chicken anti-Mouse IgG (H+L) Secondary Antibody [Alkaline Phosphatase] Summary

Immunogen
Purified Mouse IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy chains on mouse IgG and light chains on all mouse immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:5000
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.

Packaging, Storage & Formulations

Storage
Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Purity > 95% based on SDS-PAGE. Ig fraction was prepared using eggs obtained from healthy hens of US origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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