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Goat anti-Human IgG (H+L) Secondary Antibody [DyLight 488]

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Immunocytochemistry/ Immunofluorescence: Goat anti-Human IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-74945] - Human cancer cell line using Goat anti-Human IgG (H+L) Secondary Antibody [DyLight 488]. Image from ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
DyLight 488
Concentration
LYOPH

Goat anti-Human IgG (H+L) Secondary Antibody [DyLight 488] Summary

Description
Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.

Immunogen
This Goat anti-Human IgG (H+L) Secondary Antibody [DyLight 488] was developed against purified human IgG (H&L).
Specificity
Based on IEP, this Goat anti-Human IgG (H+L) Secondary Antibody [DyLight 488] reacts with heavy gamma chains on human IgG and light chains on all human immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunomicroscopy 1:20-1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-74945 in the following applications:

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Affinity purified
Reconstitution Instructions
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP1-74945) (0)

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Review for IgG (H+L) Antibody (NBP1-74945) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-74945:
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Images Ratings Applications Species Date Details
Immunocytochemistry IgG (H+L) NBP1-74945
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4
reviewed by:
Verified Customer
ICC Human 09/13/2018
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman cancer cell
SpeciesHuman
Lot26-22-020612

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Verified Customer
09/13/2018
Application: ICC
Species: Human