Rabbit F(ab')2 anti-Goat IgG (H+L) Secondary Antibody [Janelia Fluor 646] (Pre-adsorbed)

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Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 646
Format
Pre-adsorbed

Rabbit F(ab')2 anti-Goat IgG (H+L) Secondary Antibody [Janelia Fluor 646] (Pre-adsorbed) Summary

Immunogen
Purified Goat IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on goat IgG and light chains on all goat immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human, mouse, rat IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Based on IEP, this antibody reacts with and heavy (gamma) chains on goat IgG and light chains on all goat immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
>90%, by SDS-PAGE

Notes

Purity > 90% based on SDS-PAGE Small amounts of intact IgG may be present.. Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-74846JF646) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Allophycocyanin/Cy7 NBP1-74846APCCY7
DyLight 350 NBP1-74846UV
DyLight 405 NBP1-74846V
DyLight 488 NBP1-74846G
DyLight 550 NBP1-74846R
DyLight 594 NBP1-74846DL594
DyLight 650 NBP1-74846C
DyLight 680 NBP1-74846FR
DyLight 755 NBP1-74846IR
FITC NBP1-74849
HRP NBP1-74850
Janelia Fluor 549 NBP1-74846JF549
Janelia Fluor 646 NBP1-74846JF646
PE/Atto594 NBP1-74846PEATT594
PE/Cy5.5 NBP1-74846PECY55
PE/Cy7 NBP1-74846PECY7

Additional IgG (H+L) Products

Array NBP1-74846JF646

Blogs on IgG (H+L)

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Recombinant Monoclonal Antibodies

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