Rabbit anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated]

Images

 
Western Blot: Rabbit anti-Porcine IgG (H+L) Secondary Antibody [NBP1-73954] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Rabbit anti-Porcine IgG (H+L) Secondary ...read more
Western Blot: Rabbit anti-Porcine IgG (H+L) Secondary Antibody [NBP1-73954] - Lane 1: Swine IgG. Load: 100 ng per lane. Primary antibody: Porcine IgG Antibody incubated overnight at 4C. Secondary antibody: Rabbit ...read more

Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

Rabbit anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] Summary

Immunogen
Porcine IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Swine IgG and Swine Serum.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Frozen 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
Application Notes
This product is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
No Preservative
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 5.0 ml of deionized water.

Notes

Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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IgG (H+L) NBP1-73954

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