Goat anti-Equine IgG (H+L) Secondary Antibody

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Product Details

Summary
Reactivity EqSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Goat anti-Equine IgG (H+L) Secondary Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Horse IgG whole molecule
Specificity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Horse IgG and Horse Serum.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000

Reactivity Notes

Reacts with Equine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
No Preservative
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Restore with 5.0 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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