Rabbit anti-Porcine IgG (H+L) Secondary Antibody [Fluorescein]

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Western Blot: Rabbit anti-Porcine IgG (H+L) Secondary Antibody [Fluorescein] [NBP1-73903] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein swine secondary ...read more

Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB, FLISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Fluorescein
Concentration
LYOPH

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Rabbit anti-Porcine IgG (H+L) Secondary Antibody [Fluorescein] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Swine IgG whole molecule
Specificity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Swine IgG and Swine Serum.
Clonality
Polyclonal
Host
Rabbit
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000

Reactivity Notes

Reacts with Porcine.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 528, Excitation Wavelength: 495, Label Molecular Weight: 390, Restore with 2.0 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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