Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody [Biotin]

Images

 
Western Blot: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody [Biotin] [NBP1-73654] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. All samples were reduced. ...read more
Western Blot: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody [Biotin] [NBP1-73654] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. All samples were reduced. ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Guinea Pig
Conjugate
Biotin
Concentration
LYOPH

Order Details

Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody [Biotin] Summary

Immunogen
Rabbit IgG whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Guinea Pig Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Human, Goat and Mouse Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Guinea Pig
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
Application Notes
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:4,000 - 1:20,000 is suggested for this product.

Reactivity Notes

Reacts with Rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 455, Restore with 1.0 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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