Chicken anti-Human IgG (H+L) Secondary Antibody [HRP]

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Western Blot: Chicken anti-Human IgG, H/L Chains Antibody [HRP] [NBP1-73537] - Lane 1: Human IgG. Load: 100 ug per lane. Secondary antibody: Human IgG (H&L) Antibody Peroxidase conjugated at 1:1,000 for 60 min at RT. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Chicken
Conjugate
HRP
Concentration
LYOPH

Order Details

Chicken anti-Human IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
Human IgG whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken Serum, Human IgG and Human Serum.
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • ELISA 1:30000
  • Immunohistochemistry-Paraffin 1:500 - 1:2500
Application Notes
ELISA, Western Blot, Immunohistochemistry-Paraffin
Readout System

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 40,000, Restore with 1.0 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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