Goat anti-Rat IgG (H+L) Secondary Antibody [HRP]

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Western Blot: Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] [NBP1-73378] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
LYOPH

Goat anti-Rat IgG (H L) Secondary Antibody [HRP] Summary

Immunogen
Rat IgG whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000 - 1:25000
  • ELISA 1:50000 - 1:350000
  • Immunohistochemistry 1:1000 - 1:5000
Readout System

Reactivity Notes

Reacts with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 40,000, Restore with 1.0 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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